Phoenix Bio

WHAT IS A PXB-MOUSE?

PRODUCTION METHOD &
HE-STAINED LIVER IMAGES

Production Method

Transplanting the Hepatocytes

Normal human hepatocytes are transplanted via the spleen to uPA/SCID mice (immunodeficient mice with characteristics of both uPA transgenic and SCID with liver disease). Mice used for studies are aged 12 to 16 weeks and have a human hepatocyte replacement ratio of over 70 percent.

The Human Hepatocytes used

Only commercially available cryopreserved normal human hepaotcytes obtained from donors who have signed an informed consent are used for the production of PXB-mice.

HE-stained liver images of the host mice and PXB-mice

Image of PXB-mouse liver

This picture illustrates the state where the mouse hepatocytes (red) have been nearly completely replaced by the human hepatocytes with the clear cytoplasm. The hepatic lobules take normal shape through the multiplication of human hepatocytes.

 

CV: central vein
PV: portal vein

Image of host mouse (uPA+/+/SCID) liver

The mouse hepatocytes have acidophilic cytoplasm and are stained red. The hepatic cell cords are not visible due to the atrophy of the hepatocytes.

 

CV: central vein
PV: portal vein