Our PXB-mouse model is a chimeric mouse, with more than 70% of the liver replaced with normal human hepatocytes. We use the PXB-mouse to conduct study services for Drug Discovery and early Development.
The PXB-mouse is a chimeric mouse with a humanized liver that is highly repopulated by human hepatocytes.
PXB-mice are utilized in the prediction of human Metabolic Profiles, Drug Induction/Inhibition, Human PK,
Bile secretion, etc.
Using blood chemical markers such as human albumin, or by observing the histopathology of the liver directly, the PXB-mouse is able to accurately express signs of toxic effects after exposure to a compound. Human-specific toxicity can also be predicted by the human ALT1 ELISA assay, originally developed by PhoenixBio.
The Hepatitis B and C virus is efficient at infecting and proliferating within the PXB-mouse liver. The viral-loaded PXB-mouse is a powerful tool in evaluating the efficacy of anti-viral candidates, as well as for prophylaxis and infectivity of materials.
Drug candidates exhibiting both on-target and off-target effects on the hepatocytes target human-specific nucleic acid sequences or protein structures, such as RNA drugs, antibody drugs, or molecularly targeted drugs. Since human hepatocytes retain and exhibit normal function in the PXB-mouse liver, these drug candidates can be evaluated using the PXB-mice.
PXB-cells are “fresh” hepatocytes taken directly from a PXB-mouse liver, consisting of over 90% pure human hepatocytes. PXB-cells can be supplied from our large stock of PXB-mice (stable supply), which have hepatocytes from one donor (reproducibility), and can be prepared anytime you need (immediacy).
PXB-mice have over 70% of their liver repopulated with normal human hepatocytes. However, as a result of our consistent production quality, the average replacement Index of PXB-mice that are utilized for study services is 80 to 85%.
The human hepatocytes which are found in the PXB-mouse liver constitute a normal liver tissue, which secretes human albumin and human-type bile.
Over 95% of mRNAs which express in the normal human liver also express in the PXB-mouse liver. The expression of human metabolic enzymes and transporters are also observed.
HBV and HCV are well known as high species specific pathogens. PXB-mice can be efficiently infected with these viruses to develop chronic infection. Therefore, the anti-viral efficacy of drugs such as Interferon, can directly be observed in PXB-mice infected with HBV or HCV.