Phoenix Bio

PXB-mouse®

PXB-mouse® is the world's most used chimeric mouse with humanized liver

PhoenixBio supplies high-quality chimeric mice with a humanized liver that can be used to conduct studies for Drug Discovery and early Development.

What is a PXB-mouse®?

The PXB-mouse® is the chimeric mouse model with a highly humanized liver with stable human hepatocyte engraftment.

The PXB-mouse® is the chimeric mouse model with a highly humanized liver with stable human hepatocyte engraftment.

The Features of PXB-mouse®

  • Up to 95% of human hepatocyte engraftment
  • Human hepatocytes proliferate under physiologically relevant conditions
  • Histologically normal liver constitution
  • Human specific metabolism and excretion pathways
  • Permissive to HBV, HCV, and HDV
  • Humanization of lipoprotein profile

1. High replacement index

2. Normal liver constitution

Human hepatocytes in PXB-mouse liver constitute normal liver tissue, produce human albumin, and exhibit human-like biliary excretion.

3. Expression of human genes, enzymes and transporters

More than 95% of mRNA expressed in normal human liver is expressed in liver tissue, and expression of normal metabolic enzymes and transporters has also been confirmed.

4. Infection with human hepatitis viruses (HBV, HCV)

HBV and HCV are well known as high species specific pathogens. PXB-mice can be efficiently infected with these viruses to develop chronic infection. Therefore, the anti-viral efficacy of drugs such as Interferon, can directly be observed in PXB-mice infected with HBV or HCV.

Quality control criteria for PXB-mice

PXB-mouse quality control

*Average of PXB-mice used for study services and supplied to clients.

**Maximum Replacement Index of PXB-mice is up to 95% in regular production

 

Contract Study Services using PXB-mice

Case Study I: PXB-mouse® in NASH Research

PXB-mouse® has human-like lipoprotein profile

  • PXB-mouse® has human-like circulating lipoprotein cholesterol profile: LDL/HDL ratio in PXB-mice is similar to that reported in humans
  • Obeticholic acid (OCA) induced human-like changes in cholesterol profile in PXB-mice. Thus, PXB-mouse® reproduces the effects of FXR activation seen in clinical trials

Papazyan R. et al. J Lipid Res. 2018 Jun; 59(6):982-993

A novel humanized NASH liver model based on PXB-mouse®

CDAHFD Diet - PXB-mouse

  • PXB-mice develop inflammation
  • Mild fibrosis is observed after 12 weeks
  • Ballooning of human hepatocytes is seen
  • CDAHFD causes injury to human hepatocytes, as the changes of total ALT are consistent with the change of human-specific ALT-1

 

GAN Diet - PXB-mouse

  • PXB-mice develop more severe fibrosis (than with CDAHFD)
  • Large size lipid droplets, hepatocyte degeneration, proliferation and inflammation is observed
  • Ballooning in human hepatocyte area with Mallory-Denk body-like aggregates