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研究発表

研究発表

Freshly Isolated Hepatocytes from Chimeric Humanized Liver Mice for the In Vitro Assessment of Drug Metabolism, Inhibition/Induction, and Safety Studies

    Kakuni, M
    Curr Protoc. 2022 May;2(5):e429. doi: 10.1002/cpz1.429.

    Loss of mitochondrial ATPase ATAD3A contributes to non-alcoholic fatty liver disease through accumulation of lipids and damaged mitochondria

      Chen, L. Li, Y. Sottas, C. Lazaris, A. Petrillo, S. K. Metrakos, P. Li, L. Ishida, Y. Saito, T. Garza, S, Papadopoulos, V.
      J Biol Chem. 2022 May 2;298(6):102008. doi: 10.1016/j.jbc.2022.102008.

      Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1beta to downregulate PPARalpha and FOXO3

        Li, Y. Zhu, Y. Feng, S. Ishida, Y. Chiu, T. P. Saito, T. Wang, S. Ann, D. K. Ou, J. J.
        Cell Rep. 2022 Jan 25;38(4):110284. doi: 10.1016/j.celrep.2021.110284.

        HBV upregulates TRAIL-R3 expression in hepatocytes for escaping both cell apoptosis and suppression of their replication by TRAIL

          Suehiro, Y. Tsuge, M. Kurihara, M. Uchida, T. Fujino, H. Ono, A. Yamauchi, M. Makokha, G. N. Nakahara, T. Murakami, E. Abe-Chayama, H. Kawaoka, T. Miki, D. Imamura, M. Aikata, H. Hayes, C. N. Fujita, T. Chayama, K.
          J Infect Dis. 2022 Feb 28. pii: 6534478. doi: 10.1093/infdis/jiac044.

          High-throughput screening of anti-lipidemic agents using PXB-cells®, human primary hepatocytes from humanized mice livers: Assessment of lipoproteins by an enzyme-linked immunosolvent assay on apolipoproteins Apolipoprotein assay for assessing lipoprotein in hepatocyte

            Sayaka Tomatsu , Masaki Takahashi, Gen Toshima, Shiho Nakagawa, Junichiro Takahashi, Kazuya Miyashita, Kazumi Ogura, Masakazu Kakuni, Keishi Hata
            Journal of Biological Macromol …/2021; 21(2): 89-92

            Lipoprotein Z, A novel hepatotoxic lipoprotein, predicts outcome in alcoholic hepatitis

              Hu, K. Camila Perez-Matos, M. Argemi, J. Vilar, E. Shalaurova, I. Bullitt, E. Landeen, L. Sugahara, G. Deng, H. Mathur, K. Tran, S. Cai, H. He, H. Yalcin, Y. Vieira Barbosa, J. Ventura-Cots, M. Marx, K. Gad, A. P. Niezen, S. Izunza Barba, S. Lay-Hong, A. Popov, Y. V. Fricker, Z. Lai, M. Curry, M. Afdhal, N. Szabo, G. Mukamal, K. J. Sanyal, A. J. Otvos, J. D. Malik, R.
              Hepatology. 2021 Oct 18. doi: 10.1002/hep.32203.

              A Kinetic Pump Integrated Microfluidic Plate (KIM-Plate) with High Usability for Cell Culture-Based Multiorgan Microphysiological Systems

                Shinha, K. Nihei, W. Nakamura, H. Goto, T. Kawanishi, T. Ishida, N. Yamazaki, N. Imakura, Y. Mima, S. Inamura, K. Arakawa, H. Nishikawa, M. Kato, Y. Sakai, Y. Kimura, H.
                Micromachines (Basel). 2021 Aug 24;12(9):1007. doi: 10.3390/mi12091007.

                Transduction Properties of an Adenovirus Vector Containing Sequences Complementary to a Liver-Specific microRNA, miR-122a, in the 3′-Untranslated Region of the E4 Gene in Human Hepatocytes from Chimeric Mice with Humanized Liver

                  Sakurai, F. Tsukamoto, T. Ono, R. Nishimae, F. Shiota, A. Iizuka, S.Shimizu, K. Sakai, E. Ishida, Y. Tateno, C. Chayama, K. Mizuguchi, H.
                  Biol Pharm Bull. 2021;44(10):1506-1513. doi: 10.1248/bpb.b21-00394.

                  Functional changes of cocultured hepatocyte sheets subjected to continuous liver regeneration stimulation in cDNA-uPA/SCID mouse: Differences in transplantation sites

                    Miyamoto, D. Sakai, Y. Huang, Y. Yamasaki, C. Tateno, C. Hasegawa, H. Murai, T. Hara, T. Adachi, T. Soyama, A. Hidaka, M.Ito, S. Kanetaka, K. Eguchi, S.
                    Regen Ther. 2021 Mar 18;18:7-11. doi: 10.1016/j.reth.2021.02.004. eCollection 2021 Dec.

                    Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx-DDB1 Interaction

                      Sekiba, K. Otsuka, M. Ohno, M. Yamagami, M. Kishikawa, T. Suzuki, T. Ishibashi, R. Seimiya, T. Tanaka, E. Koike, K.
                      Cell Mol Gastroenterol Hepatol. 2019;7(2):297-312. doi: 10.1016/j.jcmgh.2018.10.010.

                      Infection courses, virological features and IFN-alpha responses of HBV genotypes in cell culture and animal models

                        Zhang, M. Zhang, Z. Imamura, M. Osawa, M. Teraoka, Y. Piotrowski, J. Ishida, Y. Sozzi, V. Revill, P. A. Saito, T. Chayama, K. Liang, T. J.

                        J Hepatol. 2021 Aug 4. pii: S0168-8278(21)01960-7. doi: 10.1016/j.jhep.2021.07.030.

                        Epidermal growth factor receptor is a host-entry cofactor triggering hepatitis B virus internalization

                          Iwamoto, M. Saso, W. Sugiyama, R. Ishii, K. Ohki, M. Nagamori, S. Suzuki, R. Aizaki, H. Ryo, A. Yun, J. H. Park, S. Y. Ohtani, N. Muramatsu, M. Iwami, S. Tanaka, Y. Sureau, C. Wakita, T. Watashi, K.
                          Proc Natl Acad Sci U S A. 2019 Apr 23;116(17):8487-8492. doi: 10.1073/pnas.1811064116. Epub 2019 Apr 5.

                          Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system

                            Shinohara, M. Arakawa, H. Oda, Y. Shiraki, N. Sugiura, S. Nishiuchi, T. Satoh, T. Iino, K. Leo, S. Kato, Y. Araya, K. Kawanishi, T. Nakatsuji, T. Mitsuta, M. Inamura, K. Goto, T. Shinha, K. Nihei, W.
                            Sci Rep. 2021 Mar 8;11(1):5437. doi: 10.1038/s41598-021-84861-y.

                            Geranylgeranylacetone decreases the production of hepatitis B virus-related antigen by comprehensive downregulation of mRNA transcription activity

                              Haraguchi, M. Miuma, S. Yamamoto, K. Nakao, Y. Ichikawa, T. Kanda, Y. Sasaki, R. Fukushima, M. Akazawa, Y. Miyaaki, H. Nakao, K.
                              J Gastroenterol Hepatol. 2021 Jan 4. doi: 10.1111/jgh.15394.

                              Cell-based high-throughput screening for the evaluation of reactive metabolite formation potential

                                Harada, K. Kohara, H. Yukawa, T. Matsumiya, K. Shinozawa, T.
                                Toxicol In Vitro. 2021 Aug;74:105159. doi: 10.1016/j.tiv.2021.105159. Epub 2021 Apr 3.

                                Engineered adeno-associated virus 3 vector with reduced reactivity to serum antibodies

                                  Ito, M. Takino, N. Nomura, T. Kan, A. Muramatsu, S. I.
                                  Sci Rep. 2021 Apr 29;11(1):9322. doi: 10.1038/s41598-021-88614-9.

                                  Identification of a novel long-acting 4′-modified nucleoside reverse transcriptase inhibitor against HBV

                                    Higashi-Kuwata, N. Hayashi, S. Kumamoto, H. Ogata-Aoki, H. Das, D. Venzon, D. Hattori, S. I. Bulut, H. Hashimoto, M. Otagiri, M. Takamune, N. Kishimoto, N. Davis, D. A. Misumi, S. Kakuni, M.
                                    J Hepatol. 2020 Dec 15. pii: S0168-8278(20)33843-5. doi: 10.1016/j.jhep.2020.12.006.

                                    cccDNA_Pathway_Involvement of Host ATR-CHK1 Pathway in Hepatitis B Virus Covalently Closed Circular DNA Formation

                                      Luo, J. Luckenbaugh, L. Hu, H. Yan, Z. Gao, L. Hu, J.
                                      mBio. 2020 Feb 18;11(1):e03423-19. doi: 10.1128/mBio.03423-19.

                                      Suppression of hepatitis B virus through therapeutic activation of RIG-I and IRF3 signaling in hepatocytes

                                        Sooyoung Lee, Ashish Goyal, Alan S. Perelson, Yuji Ishida, Takeshi Saito, Michael Gale, Jr.

                                        iScience. 2021 Jan 4;24(1):101969. doi: 10.1016/j.isci.2020.101969. eCollection 2021 Jan 22.

                                        PXB-cells, fresh primary hepatocytes from humanized mouse livers, exhibit nonalcoholic fatty liver like properties, including large very low density lipoprotein

                                          Sayaka Tomatsu 1, Masaki Takahashi 2, Masakazu Kakuni 2, Gen Toshima 3, Fumiko Kimura 3, Junichiro Takahashi 3, Yui Umekawa 1, Akira Sasaki 1, Keishi Hata

                                          J. Biol. Macromol., 21 (1), 51-52 (2021)