Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system

Shinohara, M. Arakawa, H. Oda, Y. Shiraki, N. Sugiura, S. Nishiuchi, T. Satoh, T. Iino, K. Leo, S. Kato, Y. Araya, K. Kawanishi, T. Nakatsuji, T. Mitsuta, M. Inamura, K. Goto, T. Shinha, K. Nihei, W.
Sci Rep. 2021 Mar 8;11(1):5437. doi: 10.1038/s41598-021-84861-y.

Geranylgeranylacetone decreases the production of hepatitis B virus-related antigen by comprehensive downregulation of mRNA transcription activity

Haraguchi, M. Miuma, S. Yamamoto, K. Nakao, Y. Ichikawa, T. Kanda, Y. Sasaki, R. Fukushima, M. Akazawa, Y. Miyaaki, H. Nakao, K.
J Gastroenterol Hepatol. 2021 Jan 4. doi: 10.1111/jgh.15394.

Cell-based high-throughput screening for the evaluation of reactive metabolite formation potential

Harada, K. Kohara, H. Yukawa, T. Matsumiya, K. Shinozawa, T.
Toxicol In Vitro. 2021 Aug;74:105159. doi: 10.1016/j.tiv.2021.105159. Epub 2021 Apr 3.

Engineered adeno-associated virus 3 vector with reduced reactivity to serum antibodies

Ito, M. Takino, N. Nomura, T. Kan, A. Muramatsu, S. I.
Sci Rep. 2021 Apr 29;11(1):9322. doi: 10.1038/s41598-021-88614-9.

Identification of a novel long-acting 4′-modified nucleoside reverse transcriptase inhibitor against HBV

Higashi-Kuwata, N. Hayashi, S. Kumamoto, H. Ogata-Aoki, H. Das, D. Venzon, D. Hattori, S. I. Bulut, H. Hashimoto, M. Otagiri, M. Takamune, N. Kishimoto, N. Davis, D. A. Misumi, S. Kakuni, M.
J Hepatol. 2020 Dec 15. pii: S0168-8278(20)33843-5. doi: 10.1016/j.jhep.2020.12.006.

cccDNA_Pathway_Involvement of Host ATR-CHK1 Pathway in Hepatitis B Virus Covalently Closed Circular DNA Formation

Luo, J. Luckenbaugh, L. Hu, H. Yan, Z. Gao, L. Hu, J.
mBio. 2020 Feb 18;11(1):e03423-19. doi: 10.1128/mBio.03423-19.

Suppression of hepatitis B virus through therapeutic activation of RIG-I and IRF3 signaling in hepatocytes

Sooyoung Lee, Ashish Goyal, Alan S. Perelson, Yuji Ishida, Takeshi Saito, Michael Gale, Jr.

iScience. 2021 Jan 4;24(1):101969. doi: 10.1016/j.isci.2020.101969. eCollection 2021 Jan 22.

PXB-cells, fresh primary hepatocytes from humanized mouse livers, exhibit nonalcoholic fatty liver like properties, including large very low density lipoprotein

Sayaka Tomatsu 1, Masaki Takahashi 2, Masakazu Kakuni 2, Gen Toshima 3, Fumiko Kimura 3, Junichiro Takahashi 3, Yui Umekawa 1, Akira Sasaki 1, Keishi Hata

J. Biol. Macromol., 21 (1), 51-52 (2021)

Role of Hepatitis B virus capsid phosphorylation in nucleocapsid disassembly and covalently closed circular DNA formation

Luo, J. Xi, J. Gao, L. Hu, J.

PLoS Pathog. 2020 Mar 30;16(3):e1008459. doi: 10.1371/journal.ppat.1008459. eCollection 2020 Mar.

Decomposition profile data analysis of multiple drug effects identifies endoplasmic reticulum stress-inducing ability as an unrecognized factor

Morita, K. Mizuno, T. Kusuhara, H.

Sci Rep. 2020 Aug 4;10(1):13139. doi: 10.1038/s41598-020-70140-9.

Utility of Three-Dimensional Cultures of Primary Human Hepatocytes (Spheroids) as Pharmacokinetic Models

Mizoi, K. Arakawa, H. Yano, K. Koyama, S. Kojima, H. Ogihara, T.

Biomedicines. 2020 Sep 23;8(10). pii: biomedicines8100374. doi: 10.3390/biomedicines8100374.

Detection of acute toxicity of aflatoxin B1 to human hepatocytes in vitro and in vivo using chimeric mice with humanized livers

Ishida, Y. Yamasaki, C. Iwanari, H. Yamashita, H. Ogawa, Y. Yanagi, A. Furukawa, S. Kojima, Y. Chayama, K. Kamiie, J. Tateno, C.

PLoS One. 2020 Sep 23;15(9):e0239540. doi: 10.1371/journal.pone.0239540.

Culture density contributes to hepatic functions of fresh human hepatocytes isolated from chimeric mice with humanized livers: Novel, long-term, functional two-dimensional in vitro tool for developing new drugs

Yamasaki, C. Ishida, Y. Yanagi, A. Yoshizane, Y. Kojima, Y. Ogawa, Y. Kageyama, Y. Iwasaki, Y. Ishida, S. Chayama, K. Tateno, C.

PLoS One. 2020 Sep 11;15(9):e0237809. doi: 10.1371/journal.pone.0237809. eCollection 2020.

2020年7月～8月に開催されました、以下の肝炎関連学会（3学会：日本消化器病学会、EASL、日本肝臓学会）にて、PXBマウス及びPXB-cells関連の研究発表がありました。

A novel evaluation method for determining drug-induced hepatotoxicity using 3D bio-printed human liver tissue

Ide, I. Nagao, E. Kajiyama, S. Mizoguchi, N.

Toxicol Mech Methods. 2020 Mar;30(3):189-196. doi: 10.1080/15376516.2019.1686795. Epub 2019 Nov 21.

Engineering Cellular Biosensors with Customizable Antiviral Responses Targeting Hepatitis B Virus

Matsunaga, S. Jeremiah, S. S. Miyakawa, K. Kurotaki, D. Shizukuishi, S. Watashi, K. Nishitsuji, H. Kimura, H. Tamura, T. Yamamoto, N. Shimotohno, K. Wakita, T. Ryo, A.

iScience. 2020 Mar 27;23(3):100867. doi: 10.1016/j.isci.2020.100867. Epub 2020 Feb 26.

Lipoprotein profile and lipid metabolism of PXB-cells((R)), human primary hepatocytes from liver-humanized mice: proposal of novel in vitro system for screening anti-lipidemic drugs

Hata, K. Sayaka, T. Takahashi, M. Sasaki, A. Umekawa, Y. Miyashita, K. Ogura, K. Toshima, G. Maeda, M. Takahashi, J. Kakuni, M.

Biomed Res. 2020;41(1):33-42. doi: 10.2220/biomedres.41.33.

Successful energy shift from glycolysis to mitochondrial oxidative phosphorylation in freshly isolated hepatocytes from humanized mice liver

Ikeyama, Y. Sato, T. Takemura, A. Sekine, S. Ito, K.

Toxicol In Vitro. 2020 Jan 25:104785. doi: 10.1016/j.tiv.2020.104785.

High-throughput screening to evaluate inhibition of bile acid transporters using human hepatocytes isolated from chimeric mice

Kohara, H. Bajaj, P. Yamanaka, K. Miyawaki, A. Harada, K. Miyamoto, K. Matsui, T. Okai, Y. Wagoner, M. Shinozawa, T.

Toxicol Sci. 2019 Nov 13. pii: 5625164. doi: 10.1093/toxsci/kfz229.

Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes

Katsuda, T. Matsuzaki, J. Yamaguchi, T. Yamada, Y. Prieto-Vila, M. Hosaka, K. Takeuchi, A. Saito, Y. Ochiya, T.

Elife. 2019 Aug 8;8. pii: 47313. doi: 10.7554/eLife.47313.