2020年7月～8月に開催されました、以下の肝炎関連学会（3学会：日本消化器病学会、EASL、日本肝臓学会）にて、PXBマウス及びPXB-cells関連の研究発表がありました。

A novel evaluation method for determining drug-induced hepatotoxicity using 3D bio-printed human liver tissue

Ide, I. Nagao, E. Kajiyama, S. Mizoguchi, N.

Toxicol Mech Methods. 2020 Mar;30(3):189-196. doi: 10.1080/15376516.2019.1686795. Epub 2019 Nov 21.

Engineering Cellular Biosensors with Customizable Antiviral Responses Targeting Hepatitis B Virus

Matsunaga, S. Jeremiah, S. S. Miyakawa, K. Kurotaki, D. Shizukuishi, S. Watashi, K. Nishitsuji, H. Kimura, H. Tamura, T. Yamamoto, N. Shimotohno, K. Wakita, T. Ryo, A.

iScience. 2020 Mar 27;23(3):100867. doi: 10.1016/j.isci.2020.100867. Epub 2020 Feb 26.

Lipoprotein profile and lipid metabolism of PXB-cells((R)), human primary hepatocytes from liver-humanized mice: proposal of novel in vitro system for screening anti-lipidemic drugs

Hata, K. Sayaka, T. Takahashi, M. Sasaki, A. Umekawa, Y. Miyashita, K. Ogura, K. Toshima, G. Maeda, M. Takahashi, J. Kakuni, M.

Biomed Res. 2020;41(1):33-42. doi: 10.2220/biomedres.41.33.

Successful energy shift from glycolysis to mitochondrial oxidative phosphorylation in freshly isolated hepatocytes from humanized mice liver

Ikeyama, Y. Sato, T. Takemura, A. Sekine, S. Ito, K.

Toxicol In Vitro. 2020 Jan 25:104785. doi: 10.1016/j.tiv.2020.104785.

High-throughput screening to evaluate inhibition of bile acid transporters using human hepatocytes isolated from chimeric mice

Kohara, H. Bajaj, P. Yamanaka, K. Miyawaki, A. Harada, K. Miyamoto, K. Matsui, T. Okai, Y. Wagoner, M. Shinozawa, T.

Toxicol Sci. 2019 Nov 13. pii: 5625164. doi: 10.1093/toxsci/kfz229.

Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes

Katsuda, T. Matsuzaki, J. Yamaguchi, T. Yamada, Y. Prieto-Vila, M. Hosaka, K. Takeuchi, A. Saito, Y. Ochiya, T.

Elife. 2019 Aug 8;8. pii: 47313. doi: 10.7554/eLife.47313.

An interferon-like small chemical compound CDM-3008 suppresses hepatitis B virus through induction of interferon-stimulated genes

Furutani, Y. Toguchi, M. Shiozaki-Sato, Y. Qin, X. Y. Ebisui, E. Higuchi, S. Sudoh, M. Suzuki, H. Takahashi, N. Watashi, K. Wakita, T. Kakeya, H. Kojima, S.

PLoS One. 2019 Jun 12;14(6):e0216139. doi: 10.1371/journal.pone.0216139. eCollection 2019.

2019年7月18日(木)～7月20日(土)に開催されました 第29回抗ウイルス療法学会学術集会にて、PXBマウス及びPXB-cells関連の研究発表がありましたのでご案内を致します。

Study of the Metabolism of New Drugs of Abuse

Kanamori, T.

Yakugaku Zasshi. 2019;139(5):699-704. doi: 10.1248/yakushi.18-00166-3.

Metabolism of Butyrylfentanyl in Fresh Human Hepatocytes: Chemical Synthesis of Authentic Metabolite Standards for Definitive Identification

Kanamori, T. Iwata, Y. T. Segawa, H. Yamamuro, T. Kuwayama, K. Tsujikawa, K. Inoue, H.

Biol Pharm Bull. 2019;42(4):623-630. doi: 10.1248/bpb.b18-00765.

PAPD5/7 Are Host Factors That Are Required for Hepatitis B Virus RNA Stabilization

Mueller, H. Lopez, A. Tropberger, P. Wildum, S. Schmaler, J. Pedersen, L. Han, X. Wang, Y. Ottosen, S. Yang, S. Young, J. A. T. Javanbakht, H.

Hepatology. 2019 Apr;69(4):1398-1411. doi: 10.1002/hep.30329. Epub 2019 Mar 1.

Prediction of Human Hepatic Clearance for Cytochrome P450 Substrates via a New Culture Method Using the Collagen Vitrigel Membrane Chamber and Fresh Hepatocytes Isolated from Liver Humanized Mice

Watari, R. Kakiki, M. Yamasaki, C. Ishida, Y. Tateno, C. Kuroda, Y. Ishida, S. Kusano, K.

Biol Pharm Bull. 2019;42(3):348-353. doi: 10.1248/bpb.b18-00582.

Chimeric mice with humanized livers demonstrate human-specific hepatotoxicity caused by a therapeutic antibody against TRAIL-receptor 2/death receptor 5

Nihira, K. Nan-Ya, K. I. Kakuni, M. Ono, Y. Yoshikawa, Y. Ota, T. Hiura, M. Yoshinari, K.

Toxicol Sci. 2018 Sep 9. pii: 5093319. doi: 10.1093/toxsci/kfy228.

Inhibitory effect of fasiglifam on hepatitis B virus infections through suppression of the sodium taurocholate cotransporting polypeptide

Nio, Y. Akahori, Y. Okamura, H. Watashi, K. Wakita, T. Hijikata, M.

Biochem Biophys Res Commun. 2018 Jun 27;501(3):820-825.

Inhibitory effect of silibinin on hepatitis B virus entry

Umetsu, T. Inoue, J. Kogure, T. Kakazu, E. Ninomiya, M. Iwata, T. Takai, S. Nakamura, T.Sano, A. Shimosegawa, T.

Biochem Biophys Rep. 2018 Mar 31;14:20-25.

A long-term culture system based on a collagen vitrigel membrane chamber that supports liver-specific functions of hepatocytes isolated from mice with humanized livers

Watari, R. Kakiki, M. Oshikata, A. Takezawa, T. Yamasaki, C. Ishida, Y. Tateno, C. Kuroda, Y. Ishida, S. Kusano, K.

J Toxicol Sci. 2018;43(8):521-529.

Inhibitory effect of Japanese rice-koji miso extracts on hepatitis A virus replication in association with the elevation of glucose-regulated protein 78 expression

Win, N. N. Kanda, T. Nakamoto, S. Moriyama, M. Jiang, X. Suganami, A. Tamura, Y. Okamoto, H. Shirasawa, H.

Int J Med Sci. 2018 Jul 30;15(11):1153-1159.

新規ready-to-use ヒト肝細胞三次元培養システム「PXB-able」の有用性の検討

赤平有希、山崎ちひろ、石田雄二、立野知世、城村友子

BIO-INDUSTRY 2018;35（3）：27-37

Use of hepatocytes isolated from a liver-humanized mouse for studies on the metabolism of drugs: application to the metabolism of fentanyl and acetylfentanyl

Kanamori, T. Togawa-Iwata, Y. Segawa, H. Yamamuro, T. Kuwayama, K. Tsujikawa, K. Inoue, H.

Forensic Toxicol. 2018;36(2):467-475.