A single hepatitis B virus genome with a reporter allows the entire viral life cycle to be monitored in primary human hepatocytes

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Sumiyadorj, A. Murai, K. Shimakami, T. Kukroki, K. Nishikawa, T. Kakuya, M. Yamada, A. Wang, Y. Ishida, A. Shirasaki, T. Kawase, S. Li, Y. Y. Okada, H. Nio, K. Kawaguchi, K. Yamashita, T. Sakai, Y. Duger, D. Mizukoshi, E. Honda, M. Kaneko, S.
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Freshly Isolated Hepatocytes from Chimeric Humanized Liver Mice for the In Vitro Assessment of Drug Metabolism, Inhibition/Induction, and Safety Studies

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/35598301/

Kakuni, M
Curr Protoc. 2022 May;2(5):e429. doi: 10.1002/cpz1.429.

Insights from a high-fat diet fed mouse model with a humanized liver

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/35533183

Saxena, R. Nassiri, M. Yin, X. M. Morral, N.
PLoS One. 2022 May 9;17(5):e0268260. doi: 10.1371/journal.pone.0268260. eCollection 2022.

Loss of mitochondrial ATPase ATAD3A contributes to non-alcoholic fatty liver disease through accumulation of lipids and damaged mitochondria

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/35513069

Chen, L. Li, Y. Sottas, C. Lazaris, A. Petrillo, S. K. Metrakos, P. Li, L. Ishida, Y. Saito, T. Garza, S, Papadopoulos, V.
J Biol Chem. 2022 May 2;298(6):102008. doi: 10.1016/j.jbc.2022.102008.

Ongoing viral replication and production of infectious virus in patients with chronic hepatitis B virus suppressed below the limit of quantitation on long-term nucleos(t)ide therapy

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/35363817

Burdette, D. L. Lazerwith, S. Yang, J. Chan, H. L. Y. Delaney Iv, W. E. Fletcher, S. P. Cihlar, T. Feierbach, B.
PLoS One. 2022 Apr 1;17(4):e0262516. doi: 10.1371/journal.pone.0262516.

Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1beta to downregulate PPARalpha and FOXO3

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/35081341

Li, Y. Zhu, Y. Feng, S. Ishida, Y. Chiu, T. P. Saito, T. Wang, S. Ann, D. K. Ou, J. J.
Cell Rep. 2022 Jan 25;38(4):110284. doi: 10.1016/j.celrep.2021.110284.

HBV upregulates TRAIL-R3 expression in hepatocytes for escaping both cell apoptosis and suppression of their replication by TRAIL

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/35226068

Suehiro, Y. Tsuge, M. Kurihara, M. Uchida, T. Fujino, H. Ono, A. Yamauchi, M. Makokha, G. N. Nakahara, T. Murakami, E. Abe-Chayama, H. Kawaoka, T. Miki, D. Imamura, M. Aikata, H. Hayes, C. N. Fujita, T. Chayama, K.
J Infect Dis. 2022 Feb 28. pii: 6534478. doi: 10.1093/infdis/jiac044.

Droplet digital PCR assay provides intrahepatic HBV cccDNA quantification tool for clinical application

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/35136096

Hayashi, S. Isogawa, M. Kawashima, K. Ito, K. Chuaypen, N. Morine, Y. Shimada, M. Higashi-Kuwata, N. Watanabe, T. Tangkijvanich, P. Mitsuya, H. Tanaka, Y.
Sci Rep. 2022 Feb 8;12(1):2133. doi: 10.1038/s41598-022-05882-9.

Simultaneous Measurement of Mouse and Human Albumin in Chimeric Mice with Humanized Livers

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/35195037/

France Landry1, Hong Shen2, Yueping Zhang2, Asako Uchiyama3, Yoshinari Miyata4, Yoshio Morikawa4, Beth Joshi2, and Petia Shipkova1
Bioanalysis. 2022 Mar;14(5):267-278. doi: 10.4155/bio-2021-0250. Epub 2022 Feb 23.

ヒト肝細胞キメラマウスを利用したB型 肝炎ウイルス/D型肝炎ウイルス共感染モデル

加国 雅和
Medical Science Digest（MSD)　2021；47（14）：26－27

Variation in Expression of Cytochrome P450 3A Isoforms and Toxicological Effects: Endo- and Exogenous Substances as Regulatory Factors and Substrates

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34719640/

Fujino, C. Sanoh, S. Katsura, T.
Biol Pharm Bull. 2021;44(11):1617-1634. doi: 10.1248/bpb.b21-00332.

Are Humanized Mouse Models Useful for Basic Research of Hepatocarcinogenesis through Chronic Hepatitis B Virus Infection?

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/34696350

Masataka Tsuge
Viruses. 2021 Sep 24;13(10). pii: v13101920. doi: 10.3390/v13101920.

High-throughput screening of anti-lipidemic agents using PXB-cells®, human primary hepatocytes from humanized mice livers: Assessment of lipoproteins by an enzyme-linked immunosolvent assay on apolipoproteins Apolipoprotein assay for assessing lipoprotein in hepatocyte

• Pub Med URL https://www.jstage.jst.go.jp/article/jbm/21/2/21_89/_article/-char/ja/

Sayaka Tomatsu , Masaki Takahashi, Gen Toshima, Shiho Nakagawa, Junichiro Takahashi, Kazuya Miyashita, Kazumi Ogura, Masakazu Kakuni, Keishi Hata
Journal of Biological Macromol …/2021; 21(2): 89-92

Elucidation of the species differences of epyrifenacil-induced hepatotoxicity between mice and humans by mass spectrometry imaging analysis in chimeric mice with humanized liver

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34853245/

Matsunaga, K. Abe, J. Ogata, K. Fukunaga, S. Kitamoto, S.
J Toxicol Sci. 2021;46(12):601-609. doi: 10.2131/jts.46.601.

Estimating Drug Efficacy with a Diet-Induced NASH Model in Chimeric Mice with Humanized Livers

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34829876/

Kisoh, K. Sugahara, G. Ogawa, Y. Furukawa, S. Ishida, Y. Okanoue, T. Kohara, M. Tateno, C.
Biomedicines. 2021 Nov 9;9(11):1647. doi: 10.3390/biomedicines9111647.

Utility of Gottingen minipigs for the prediction of human pharmacokinetic profiles after intravenous drug administration

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/34710650

Ding, N. Yamamoto, S. Chisaki, I. Nakayama, M. Matsumoto, S. I. Hirabayashi, H.
Drug Metab Pharmacokinet. 2021 Dec;41:100408. doi: 10.1016/j.dmpk.2021.100408. Epub 2021 Jun 9.

Lipoprotein Z, A novel hepatotoxic lipoprotein, predicts outcome in alcoholic hepatitis

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34662439/

Hu, K. Camila Perez-Matos, M. Argemi, J. Vilar, E. Shalaurova, I. Bullitt, E. Landeen, L. Sugahara, G. Deng, H. Mathur, K. Tran, S. Cai, H. He, H. Yalcin, Y. Vieira Barbosa, J. Ventura-Cots, M. Marx, K. Gad, A. P. Niezen, S. Izunza Barba, S. Lay-Hong, A. Popov, Y. V. Fricker, Z. Lai, M. Curry, M. Afdhal, N. Szabo, G. Mukamal, K. J. Sanyal, A. J. Otvos, J. D. Malik, R.
Hepatology. 2021 Oct 18. doi: 10.1002/hep.32203.

A Kinetic Pump Integrated Microfluidic Plate (KIM-Plate) with High Usability for Cell Culture-Based Multiorgan Microphysiological Systems

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34577652/

Shinha, K. Nihei, W. Nakamura, H. Goto, T. Kawanishi, T. Ishida, N. Yamazaki, N. Imakura, Y. Mima, S. Inamura, K. Arakawa, H. Nishikawa, M. Kato, Y. Sakai, Y. Kimura, H.
Micromachines (Basel). 2021 Aug 24;12(9):1007. doi: 10.3390/mi12091007.

Hexa Histidine-Tagged Recombinant Human Cytoglobin Deactivates Hepatic Stellate Cells and Inhibits Liver Fibrosis by Scavenging Reactive Oxygen Species

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/33576020

Dat, N. Q. Thuy, L. T. T. Hieu, V. N. Hai, H. Hoang, D. V. Thi Thanh Hai, N. Thuy, T. T. V. Komiya, T. Rombouts, K. Dong, M. P. Hanh, N. V. Hoang, T. H. Sato-Matsubara, M. Daikoku, A. Kadono, C. Oikawa, D. Yoshizato, K. Tokunaga, F. Pinzani, M. Kawada, N.
Hepatology. 2021 Jun;73(6):2527-2545. doi: 10.1002/hep.31752. Epub 2021 May 22.

Transduction Properties of an Adenovirus Vector Containing Sequences Complementary to a Liver-Specific microRNA, miR-122a, in the 3′-Untranslated Region of the E4 Gene in Human Hepatocytes from Chimeric Mice with Humanized Liver

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34602559/

Sakurai, F. Tsukamoto, T. Ono, R. Nishimae, F. Shiota, A. Iizuka, S.Shimizu, K. Sakai, E. Ishida, Y. Tateno, C. Chayama, K. Mizuguchi, H.
Biol Pharm Bull. 2021;44(10):1506-1513. doi: 10.1248/bpb.b21-00394.