Simultaneous Measurement of Mouse and Human Albumin in Chimeric Mice with Humanized Livers

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/35195037/

France Landry1, Hong Shen2, Yueping Zhang2, Asako Uchiyama3, Yoshinari Miyata4, Yoshio Morikawa4, Beth Joshi2, and Petia Shipkova1
Bioanalysis. 2022 Mar;14(5):267-278. doi: 10.4155/bio-2021-0250. Epub 2022 Feb 23.

ヒト肝細胞キメラマウスを利用したB型 肝炎ウイルス/D型肝炎ウイルス共感染モデル

加国 雅和
Medical Science Digest（MSD)　2021；47（14）：26－27

Variation in Expression of Cytochrome P450 3A Isoforms and Toxicological Effects: Endo- and Exogenous Substances as Regulatory Factors and Substrates

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34719640/

Fujino, C. Sanoh, S. Katsura, T.
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Are Humanized Mouse Models Useful for Basic Research of Hepatocarcinogenesis through Chronic Hepatitis B Virus Infection?

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/34696350

Masataka Tsuge
Viruses. 2021 Sep 24;13(10). pii: v13101920. doi: 10.3390/v13101920.

High-throughput screening of anti-lipidemic agents using PXB-cells®, human primary hepatocytes from humanized mice livers: Assessment of lipoproteins by an enzyme-linked immunosolvent assay on apolipoproteins Apolipoprotein assay for assessing lipoprotein in hepatocyte

• Pub Med URL https://www.jstage.jst.go.jp/article/jbm/21/2/21_89/_article/-char/ja/

Sayaka Tomatsu , Masaki Takahashi, Gen Toshima, Shiho Nakagawa, Junichiro Takahashi, Kazuya Miyashita, Kazumi Ogura, Masakazu Kakuni, Keishi Hata
Journal of Biological Macromol …/2021; 21(2): 89-92

Elucidation of the species differences of epyrifenacil-induced hepatotoxicity between mice and humans by mass spectrometry imaging analysis in chimeric mice with humanized liver

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34853245/

Matsunaga, K. Abe, J. Ogata, K. Fukunaga, S. Kitamoto, S.
J Toxicol Sci. 2021;46(12):601-609. doi: 10.2131/jts.46.601.

Estimating Drug Efficacy with a Diet-Induced NASH Model in Chimeric Mice with Humanized Livers

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34829876/

Kisoh, K. Sugahara, G. Ogawa, Y. Furukawa, S. Ishida, Y. Okanoue, T. Kohara, M. Tateno, C.
Biomedicines. 2021 Nov 9;9(11):1647. doi: 10.3390/biomedicines9111647.

Utility of Gottingen minipigs for the prediction of human pharmacokinetic profiles after intravenous drug administration

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/34710650

Ding, N. Yamamoto, S. Chisaki, I. Nakayama, M. Matsumoto, S. I. Hirabayashi, H.
Drug Metab Pharmacokinet. 2021 Dec;41:100408. doi: 10.1016/j.dmpk.2021.100408. Epub 2021 Jun 9.

Lipoprotein Z, A novel hepatotoxic lipoprotein, predicts outcome in alcoholic hepatitis

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34662439/

Hu, K. Camila Perez-Matos, M. Argemi, J. Vilar, E. Shalaurova, I. Bullitt, E. Landeen, L. Sugahara, G. Deng, H. Mathur, K. Tran, S. Cai, H. He, H. Yalcin, Y. Vieira Barbosa, J. Ventura-Cots, M. Marx, K. Gad, A. P. Niezen, S. Izunza Barba, S. Lay-Hong, A. Popov, Y. V. Fricker, Z. Lai, M. Curry, M. Afdhal, N. Szabo, G. Mukamal, K. J. Sanyal, A. J. Otvos, J. D. Malik, R.
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A Kinetic Pump Integrated Microfluidic Plate (KIM-Plate) with High Usability for Cell Culture-Based Multiorgan Microphysiological Systems

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34577652/

Shinha, K. Nihei, W. Nakamura, H. Goto, T. Kawanishi, T. Ishida, N. Yamazaki, N. Imakura, Y. Mima, S. Inamura, K. Arakawa, H. Nishikawa, M. Kato, Y. Sakai, Y. Kimura, H.
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Hexa Histidine-Tagged Recombinant Human Cytoglobin Deactivates Hepatic Stellate Cells and Inhibits Liver Fibrosis by Scavenging Reactive Oxygen Species

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/33576020

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Transduction Properties of an Adenovirus Vector Containing Sequences Complementary to a Liver-Specific microRNA, miR-122a, in the 3′-Untranslated Region of the E4 Gene in Human Hepatocytes from Chimeric Mice with Humanized Liver

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34602559/

Sakurai, F. Tsukamoto, T. Ono, R. Nishimae, F. Shiota, A. Iizuka, S.Shimizu, K. Sakai, E. Ishida, Y. Tateno, C. Chayama, K. Mizuguchi, H.
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Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx-DDB1 Interaction

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/30704981

Sekiba, K. Otsuka, M. Ohno, M. Yamagami, M. Kishikawa, T. Suzuki, T. Ishibashi, R. Seimiya, T. Tanaka, E. Koike, K.
Cell Mol Gastroenterol Hepatol. 2019;7(2):297-312. doi: 10.1016/j.jcmgh.2018.10.010.

PXBマウス関連の学術論文(NASH病態モデル動物)のご紹介：株式会社フェニックスバイオ

弊社研究員(喜早)のPXBマウス関連の学術論文が発表されましたのでご紹介申し上げます。

【詳細】
掲載雑誌名：Biomedicines 2021, 9, 1647. ; https://doi.org/10.3390/biomedicines9111647
論文名：Estimating Drug Efficacy with a Diet-Induced NASH Model in Chimeric Mice with Humanized Livers.
著者：Keishi Kisoh, et. al.（株式会社フェニックスバイオ）

概要：
非アルコール性脂肪性肝疾患/肝炎（NAFLD/NASH）は生活習慣病と密接にかかわる疾患で先進国を中心にその患者数が増加傾向にあり、肝硬変や肝がんの原因となる重大な健康障害の1つです。現在、NASHに対して有用な治療薬は存在せず、多くの製薬会社等でその開発が進められています。今回我々は、東京都医学総合研究所および大阪府済生会吹田病院との共同研究により、ヒト肝細胞キメラマウス（PXBマウス）にコリン欠乏メチオニン減量高脂肪食（CDAHFD）を摂餌することでヒトNASHモデルの作製に成功しました。本モデルは、脂肪肝による肝障害から線維化までの一連のNASH病態を発症するのに加え、バルーニングやマロリーデング小体＊といったヒトNASH患者で見られる所見と同様の形態の肝細胞変性を示しておりました。さらに、本モデルにPPARa/d agonistのElafibranorを処置したところ、治験と同様の結果が得られました。以上の内容は、PXBマウスがNASHの病態解明や創薬研究に寄与できることを示す重要な結果であると考えています。

＊バルーニング・マロリーデング小体
従来のマウスモデルでは、脂肪化した肝細胞との判別が難しかったがPXBマウスでは判別が容易で病態のマーカーとして評価が可能である。

Bile acid dysmetabolism in the gut-microbiota-liver axis under hepatitis C virus infection

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/34411400

Inoue, T. Funatsu, Y. Ohnishi, M. Isogawa, M. Kawashima, K. Tanaka, M. Moriya, K. Kawaratani, H. Momoda, R. Iio, E. Nakagawa, H. Suzuki, Y. Matsuura, K. Fujiwara, K. Nakajima, A. Yoshiji, H. Nakayama, J. Tanaka, Y.

Liver Int. 2021 Aug 19. doi: 10.1111/liv.15041.

HBx-induced degradation of Smc5/6 complex impairs homologous recombination-mediated repair of damaged DNA

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/34478763

Sekiba, K. Otsuka, M. Funato, K. Miyakawa, Y. Tanaka, E. Seimiya, T. Yamagami, M. Tsutsumi, T. Okushin, K. Miyakawa, K. Ryo, A. Koike, K.

J Hepatol. 2021 Aug 25. pii: S0168-8278(21)02005-5. doi: 10.1016/j.jhep.2021.08.010.

Infection courses, virological features and IFN-alpha responses of HBV genotypes in cell culture and animal models

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/34363922

Zhang, M. Zhang, Z. Imamura, M. Osawa, M. Teraoka, Y. Piotrowski, J. Ishida, Y. Sozzi, V. Revill, P. A. Saito, T. Chayama, K. Liang, T. J.

J Hepatol. 2021 Aug 4. pii: S0168-8278(21)01960-7. doi: 10.1016/j.jhep.2021.07.030.

Chimeric Mouse With Humanized Liver Is an Appropriate Animal Model to Investigate Mode of Action for Porphyria-Mediated Hepatocytotoxicity

• Pub Med URL https://pubmed.ncbi.nlm.nih.gov/34238059/

Toxicol Pathol. 2021 Jul 9:1926233211027474. doi: 10.1177/01926233211027474.

Eguchi, A. Fukunaga, S. Ogata, K. Kushida, M. Asano, H. Cohen, S. M. Sukata, T.

Application of humanized mice to toxicology studies: Evaluation of the human relevance of the mode of action for rodent liver tumor formation by activators of the constitutive androstane receptor (CAR)

• Pub Med URL https://doi.org/10.1293/tox.2021-0027

Journal Toxicologic Pathology 2021; 34(4): 283-297. doi: https://doi.org/10.1293/tox.2021-0027

Tomoya Yamada

Critical evaluation of the human relevance of the mode of action for rodent liver tumor formation by activators of the constitutive androstane receptor (CAR)

• Pub Med URL https://www.ncbi.nlm.nih.gov/pubmed/34264181

Yamada, T. Cohen, S. M. Lake, B. G.

Crit Rev Toxicol. 2021;51(5):373-394. doi:10.1080/10408444.2021.1939654. Epub 2021 Jul 15.